Prof. Melanie Sadono Djamil
Melanie Sadono Djamil is a Professor in Biochemistry Biomolecular and Oral Biology at Faculty of Dentistry, Trisakti University, Indonesia. She is the Head of Indonesia Dental Council - Indonesia Medical Council with a main responsibility in improving continuous dental education, registration of dentists in Indonesia and overall development of the dental profession. She is the past President of IADR-SEA Division from 2015 to 2017. She has been conducting research mainly on utilizing natural agents in dentistry. Prof. Djamil has received an honorary PhD in Oral Health Science, Faculty of Dentistry, Thammasat University, Bangkok, Thailand.
Title: Pandanus conoideus Lam. extract on HONE-1 cell line apoptosis induction
Malignancy in head and neck areas considering high and become global issues. Pandanus conoideus Lam., as a chemo preventive compound that can enhance the immune response. Apoptosis intrinsic pathway, involves non-receptor induction from mitochondria. Cyt C with several cofactors activates caspase 3. Western blot analysis was used to test Pandanus conoideus Lam, which was immersed in antibody until a specific protein was formed. Based on the western blot analysis of Pandanus conoideus Lam on cell line HONE-1 activation of the target protein was proven at a concentration of 40 g/mL. Conclusion Pandanus conoideus Lam is able to induce apoptosis dependent on concentration and time of exposure, via activation of caspase-3 in cells line HONE-1.